[theholisticgoat]

Repeated dose (28-day) administration of silver nanoparticles of varied size and coating does not significantly alter the indigenous murine gut microbiome

Abstract

Silver nanoparticles (AgNPs) have been used as antimicrobials in a number of applications, including topical wound dressings and coatings for consumer products and biomedical devices. Ingestion is a relevant route of exposure for AgNPs, whether occurring unintentionally via Ag dissolution from consumer products, or intentionally from dietary supplements. AgNP have also been proposed as substitutes for antibiotics in animal feeds. While oral antibiotics are known to have significant effects on gut bacteria, the antimicrobial effects of ingested AgNPs on the indigenous microbiome or on gut pathogens are unknown. In addition, AgNP size and coating have been postulated as significantly influential towards their biochemical properties and the influence of these properties on antimicrobial efficacy is unknown. We evaluated murine gut microbial communities using culture-independent sequencing of 16S rRNA gene fragments following 28 days of repeated oral dosing of well-characterized AgNPs of two different sizes (20 and 110 nm) and coatings (PVP and Citrate). Irrespective of size or coating, oral administration of AgNPs at 10 mg/kg body weight/day did not alter the membership, structure or diversity of the murine gut microbiome. Thus, in contrast to effects of broad-spectrum antibiotics, repeat dosing of AgNP, at doses equivalent to 2000 times the oral reference dose and 100-400 times the effective in vitro anti-microbial concentration, does not affect the indigenous murine gut microbiome.

https://pubmed.ncbi.nlm.nih.gov/26525505/

Repeated-dose-28-day-administration-of-silver-nanoparticles-of-varied-size-and-coating-does-not-significantly-alter-the-indigenous-murine-gut-microbiome-PubMed.pdf 227 KB PDF - Click to view

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[theholisticgoat]

Okay, my gallon colloidal silver brewer.

-This cord or one with the same v and mA: https://www.amazon.com/KFD-Adapter-0957-2242-0957-2269-OfficeJet/dp/B01AFN5EAM

-A one-gallon glass jar with straight sides.

-(2) 12″ sections of silver wire. I bought 4′ coils so I had spares: https://www.goldenstatesilver.com/products/14-gauge-wire-coils

-(2) alligator clips

-Electrical tape

The double cord coming from the box that plugs into the printer, split it apart and attach an alligator clip to each wire. Wrap in electrical tape.

Fold 12″ silver wire over the top of the jar with .5″-1″ length on the outside so it stays put, twice. This is where you attach your clips. The bottom of the wire, curve it up so it doesn’t touch the bottom, about 1″ or so curved space at the bottom. Once placed, bend slightly so the rods follow the curve of the jar because it is in the space between the rods that silver is brewed.

Add distilled water and brew. You can take a shortcut by adding a quart of colloidal silver to act as a conductor so to speak for the rods. When we add a quart of silver, brew time is around 80 minutes right now but it varies based on temperature so shorter in the heat of summer and longer in the cold. My mentor said to brew until just slightly yellow, then back off 20 minutes and you know how much time you need. It’s really hard to mess this process up, but properly brewed will have a slight metallic taste most folks can taste (not all).

Switch alligator clips every session to ensure even wear.

I’m not an expert and can only help you as far as my own experience, but this is what worked for me and I’ve used this setup for 3 or so years now.