Reply To: Oregano Essential Oil

  • theholisticgoat

    October 8, 2023 at 10:18 am

    Effects of dietary oregano essential oil supplementation on growth performance, intestinal antioxidative capacity, immunity, and intestinal microbiota in yellow-feathered chickens


    Essential oils are plant-derived aromatic volatile oils, and they contain bioactive compounds that have been shown to
    improve poultry nutrition. In this study, we investigated the effects of oregano essential oil (OEO) on intestinal antioxidative
    capacity, immunity, and gut microbiota of young yellow-feathered chickens. A total of nine hundred and sixty 1-d-old
    female Qingyuan partridge chickens were randomly allocated to four treatment groups with six replicates of 40 birds each,
    and the feeding trial was lasted for 30 d. The controls were fed on a basal diet without in-feed antibiotics; the birds in the
    antibiotic group were fed the basal diet supplemented with 20 mg/kg virginiamycin; the remaining birds were fed the basal
    diet containing 150 or 300 mg/kg OEO, respectively. Dietary supplementation with 150 or 300 mg/kg OEO increased average
    daily feed intake (P = 0.057) and average daily gain (P < 0.05). The activities of glutathione peroxidase and total antioxidative
    capacity in plasma, jejuna, and ileal mucosa were increased by OEO supplementation (P < 0.05), with a trend of lower jejunal
    content of malonaldehyde (P = 0.062). Moreover, dietary OEO increased the content of secretory immunoglobulin A (P = 0.078)
    and the relative expression of Claudin 1, Mucin 2, and Avain beta-defensin 1 in ileum (P < 0.05). Sequencing data of 16S
    rRNA indicated that dietary OEO increased the relative abundance of Firmicutes phylum, and Clostridium and Lactobacillus
    genera, and decreasing that of Romboutsia. Functional analyses indicated that microbial amino sugar and nucleotide sugar
    metabolism, replication, and repair systems were higher in OEO groups than those of controls and antibiotic treatment. In
    conclusion, dietary supplementation with OEO enhanced growth performance, alleviated local oxidative stress in intestine,
    improved production of natural antibodies, and favorably modulated intestinal microbiota composition.